Simultaneous Estimation Of Drugs Used For Treatment Of Erectile Dysfunctioning In Bulk And In Their Combined Dosage Form.
Patel Vandana, Vaswani Mamta*

Department of Quality Assurance, Babaria Institute of Pharmacy, BITs edu campus, Varnama-391240, Vadodara, Gujarat, India. Email id: Contact no. : +919033368185

A simple, precise and accurate UV Spectrophotometric method (Absorbance Ratio method) was developed for simultaneous estimation of drugs used for treatment of Erectile Dysfunctioning (Sildenafil citrate and Dapoxetine hydrochloride). The developed method was validated as per ICH guidelines Q2-R1. In this method, the spectra of Sildenafil citrate and Dapoxetine hydrochloride were taken and wavelengths 239.0 (i.e. Iso-Absorptive point) and 292.0 nm (λmax of Sildenafil citrate) were selected for the formation of the Q-Ratio equations. The linearity for Sildenafil citrate was found to be 4-20μg/ml and 5-25μg/ml for Dapoxetine hydrochloride. The developed method was validated as per ICH guideline and was found to be precise with % RSD < 2 and accuracy was found to be in range. The results clearly indicated that the developed UV method had its potential utility and would be suitable for simultaneous estimation of both the drug

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